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Image Search Results
Journal: Nucleic Acids Research
Article Title: Saccharomyces cerevisiae HMO1 interacts with TFIID and participates in start site selection by RNA polymerase II
doi: 10.1093/nar/gkm1068
Figure Lengend Snippet: The effect of Δ hmo1 on start site selection of class II genes in sua7 mutants. ( A ) Transcriptional start sites of RPS5 in Δ hmo1 and/or sua7 mutants. Total RNA (20 μg) from strains containing the alleles indicated at the top was isolated 2 h after a temperature shift to 37°C from 30°C before being subjected to primer extension analysis. The position of a major transcriptional start site (C at −37 numbered relative to the A (+1) of the start codon ATG) is indicated by the asterisk. ( B ). Transcriptional start sites of RPL32 in Δ hmo1 and/or sua7 mutants. Primer extension analysis was done as described in A. A major transcriptional start site at −365 is indicated by the asterisk. ( C ) Transcriptional start sites of RPS31 in Δ hmo1 and/or sua7 mutants. Primer extension analysis was done as described in A. A major transcriptional start site at −57 is indicated by the asterisk. ( D ) Transcriptional start sites of RPL10 in Δ hmo1 and/or sua7 mutants. Primer extension analysis was done as described in A. A major transcriptional start site at −21 is indicated by the asterisk. ( E ) Each lane of the electropherogram shown in A was scanned and quantified by densitometry (Multi Gauge ver.3.0, Fuji Film) ( SUA7 , left panel; sua7-R78C , center panel; sua7-K190E , right panel). The solid and broken lines represent the results obtained from HMO1 and Δ hmo1 strains, respectively. Asterisks indicate the peaks that correspond to the major transcriptional start sites described in A. The upstream regions were expanded and are shown in the lower panels to make the differences between HMO1 and Δ hmo1 strains more evident. ( F ) Each lane of the electropherogram shown in B was scanned and presented as described in E. ( G ) Each lane of the electropherogram shown in C was scanned and presented as described in E. ( H ) Each lane of the electropherogram shown in D was scanned and presented as described in E.
Article Snippet: A major transcriptional start site at −21 is indicated by the asterisk. ( E ) Each lane of the electropherogram shown in A was scanned and quantified by
Techniques: Selection, Isolation